General description
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Biochem/physiol Actions
The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
Physical form
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | FITC conjugate |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | C-11, monoclonal |
| form | buffered aqueous solution |
| species reactivity | (Mammalian) |
| concentration | 1 mg/ml |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
