General description
Signal transducer and activator of transcription 1-alpha/beta (UniProt: P42224, also known as Transcription factor ISGF-3 components p91/p84) is encoded by the STAT1 gene (Gene ID: 6772) in human. STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling proteins. STAT1 is activated by many different ligands including interferons, interleukins, and growth factors. It exists in two forms, the 91 kDa STAT1 α and 84 kDa STAT1 β , which are encoded by the same gene with splicing variant. Following ligand binding to cell surface receptors, signaling via protein kinases leads to activation of JAK kinases and tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and enter the nucleus where it activates expression of multiple proinflammatory genes, including inducible nitric oxide synthase (iNOS) and cyclooxygenase. Loss of STAT1 activity can lead to early fatal infections and increased susceptibility to severe mycobacterial and viral infections. Phosphorylation on serine 727 by several kinases including MAPK14, ERK1/2 and CAMKII following on IFN-g stimulation, regulates STAT1 transcriptional activity. This phosphorylation also promotes sumoylation though increasing interaction with PIAS. Clone 3G14 detects both alpha and beta isoforms of STAT1. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of human STAT1.
Application
Anti-STAT1 ( & β) clone 3G14 ZooMAb, Cat. No. ZRB1497, is a recombinant Rabbit monoclonal antibody that specifically detects STAT1 ( & β) and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected STAT1 (α & β) in RAW264.7 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected STAT1 ( α & β) in HeLa cells treated with IFN-gamma.
Affinity Binding Assay: A representative lot of this antibody bound STAT1 peptide with a KD of 1.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 3G14 is a ZooMAb rabbit recombinant monoclonal antibody that detects STAT1 alpha and beta isoforms. It targets an epitope within 23 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3G14, monoclonal,recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 83.04 kDa,calculated mol wt 87.33 kDa,observed mol wt 84 kDa,observed mol wt 91 kDa |
| species reactivity | human, mouse |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | P42224 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... STAT1(6772) mouse ... Stat1(20846) |
