产品介绍
Product Description
General description
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Application
An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.
Physical form
Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative
Preparation Note
TPCK-treated
Other Notes
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).
技术参数
Specifications
| biological source | bovine pancreas (trypsin) |
| Quality Segment | 200 |
| form | buffered aqueous suspension |
| concentration | ≥15 units/mL (packed gel) |
| extent of labeling | ≥15 units per mL (packed gel) |
| matrix | beaded agarose |
| storage temp. | 2-8°C |
