General description
Recoverin (UniProt: P35243; also known as Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known s RCV1) gene (Gene ID: 5957) in human. Recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins that is expressed in retinal photoreceptors and midget cone bipolar cells. It unctions as a calcium sensor that regulates rhodopsin phosphorylation through inhibition of rhodopsin kinase. It contains four EF hand motifs (aa 25-60; 61-96; 97-132; 147-182) and a myristoyl chain on the N-terminus. It has a high affinity (aa 110-121) and a low affinity (aa 74-85) calcium binding site. Recoverin undergoes a conformational change upon calcium binding in darkness and subsequently it attaches to the disc membrane in photoreceptor outer segment. This in turn allows binding of G protein-coupled receptor kinases (GRK). Calcium-bound Recoverin is shown to inhibit G-protein-coupled receptor kinase (GRKs) in darkness, which prevents visual pigment phosphorylation. Under bright light condition, calcium dissociates from Recoverin and the protein translocates to the inner segment. The released GRK can phosphorylate and deactivate the light activated visual pigment. Mutations in Recoverin have been linked to retinal dysfunction associated with early-stage diabetes and cancer-associated retinopathy. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zang. J., and Neuhauss, SCF (2018). Front. Mol. Neurosci 11:473).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
His-tagged recombinant full-length human Recoverin.
Application
Anti-Recoverin, clone 1J23 ZooMAb , Cat. No. ZRB1107, is a recombinant rabbit monoclonal antibody that specifically targets Recoverin and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Recoverin in ARPE-19 cells treated with 3 uM fenretinide for 7 days.
Immunofluorescence Analysis: A 1:1,250 dilution from a representative lot detected Recoverin in human retina tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:1,250 dilution from a representative lot detected Recoverin in human retina tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1J23 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects human Recoverin.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1J23, recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 23.13 kDa,observed mol wt ~21 kDa |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable,immunofluorescence: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable (peptide) |
| isotype | IgG |
| UniProt accession no. | P35243 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... RCVRN(5957) |
