General description
Metabotropic glycine receptor (UniProt: Q5T848; also known as mGlyR, G-protein coupled receptor 158) is encoded by the GPR158 (also known as KIAA1136) gene (Gene ID: 57512) in human. GPR158 is a member of the G protein-coupled receptor (GPCR) superfamily. It is characterized by seven transmembrane domains, an extracellular N-terminus, and a large intracellular C-terminus. GPR158 is classified as a metabotropic receptor for glycine, although it can also respond to other ligands. Unlike ionotropic receptors, which directly mediate ion flux across membranes, GPR158 influences cellular processes through second messenger systems. It has been implicated in various signaling pathways, including those involved in cell growth and synaptic plasticity. GPR158 is known to interact with G proteins and potentially other signaling molecules, modulating neurotransmitter release and neuronal excitability. GPR158 is highly expressed in the brain, particularly in the cortex and hippocampus. Within neurons, GPR158 is predominantly localized to the post-synaptic density. It is also expressed in other tissues, including the eye and reproductive organs. Dysregulation of GPR158 has been associated with several neurological and psychiatric disorders. Its overexpression has been linked to major depressive disorder, where it is thought to affect the glutamatergic system and synaptic plasticity. In addition, GPR158 have been implicated in the pathophysiology of glaucoma and certain reproductive disorders. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human GPR158.
Application
Quality Control Testing
Evaluated by Western Blotting in human prostate tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GPR158 in human prostate tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected His-tagged recombinant fragment of human GPR158.
Affinity Binding Assay: A representative lot of this antibody bound GPR158 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GPR158 in mouse brain and human gallbladder tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GPR158 in C6 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2D19 is a ZooMAb® rabbit recombinant monoclonal antibody that detects GPR158. It targets an epitope within 21 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2D19, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 135.49 kDa,observed mol wt ~150 kDa |
| purified by | using Protein A |
| species reactivity | human, rat, mouse |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgGκ |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_065803 |
| UniProt accession no. | Q5T848 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... GPR158(57512) |
