General description
Goat Anti-Rabbit IgG(Fcγ) Biotin Alpaca Heavy chain only Antibody is designed to specifically detect Fc region of rabbit IgG. It is a monoclonal, recombinant, goat IgG Fc fused single domain antibody to Fc region of rabbit IgG coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Goat Anti-Rabbit IgG(Fcγ) Biotin Alpaca Heavy chain only Antibody reacts with the Fc fragment of rabbit IgG selectively but not with mouse, human, cynomolgus, rat, goat IgG.
Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Immunogen
Recombinant Alpaca single variable domain secondary nano antibody
Biochem/physiol Actions
Fc region of Rabbit IgG
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Packaging
100 μg in Glass bottle
Physical form
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
Preparation Note
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C
Other Notes
No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG
| biological source | alpaca |
| recombinant | expressed in HEK 293 cells (Human cell line) |
| conjugate | biotin |
| antibody form | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | recombinant monoclonal |
| form | lyophilized solid |
| mol wt | 100 kDa |
| species reactivity | rabbit, Alpaca |
| technique(s) | ELISA: 1:10000-1:50000,western blot: 1:10000-1:50000 |
| size | 0.1 mL |
| color | colorless |
| isotype | VHH |
| shipped in | wet ice |
| storage temp. | 2-8°C |
