General description
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Immunogen
Murine peripheral blood leukocytes
Biochem/physiol Actions
The rat monoclonal antibody EM-05 reacts with an extracellular epitope of murine CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
Physical form
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | Peridinin-Chlorophyll-Protein Complex conjugate |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | EM-05, monoclonal |
| form | buffered aqueous solution |
| species reactivity | mouse |
| concentration | 0.5 mg/ml |
| technique(s) | flow cytometry: suitable |
| isotype | IgG |
| UniProt accession no. | Q8C6Q7 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... Ptprc(19264) |
