ExoPolyzyme，1YY0，MBD0064-1VL，Sigma

General description

Biofilms are complex structures, which adhere to surfaces and comprise of one or more types of bacteria and fungi. They form an extracellular matrix (ECM) which is generally composed of secreted polymers such as exopolysaccharides (EPS), extracellular DNA (eDNA), peptides and lipids. ExoPolyzyme is a mixture of seven enzymes that break down different components residing in ECM, such as EPS’s and lipids. Each of these enzymes has been previously used to inhibit or remove biofilm. The original development was in collaboration with Scott Tighe and the Extreme Microbiome Project.

Application

ExoPolyzme can be used in biofilm research and as a gentler method for removing the EPS and lipids from the ECM in metagenomic protocols, providing better access to the bacterial cells within the biofilm and eDNA.

Features and Benefits

• Mixture of seven enzymes that break down different components residing in ECM, such as EPS’s and lipids
• Gently removes the EPS and lipids from the ECM in metagenomic protocols
• Allows better access to the bacterial cells within the biofilm and eDNA

Other Notes

The components in ExoPolyzyme (MBD0064) are:

• α-Amylase
• Cellulase
• β-Glucosidase
• Lyticase
• Alginate lyase
• Lipase
• Hemicellulase