产品介绍
Product Description
General description
Human HDAC2, GenBank Accession No. Q92769, full length with C-terminal His tag, MW = 60 kDa, expressed in baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN® 20, and 20% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
Legal Information
TWEEN is a registered trademark of Croda International PLC
技术参数
Specifications
| biological source | human |
| recombinant | expressed in baculovirus infected insect cells |
| assay | ≥80% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 60 kDa |
| packaging | pkg of 50 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | >0.02 mg/mL |
| NCBI accession no. | Q92769 |
| UniProt accession no. | Q92769 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... HDAC2(3066) |
