产品介绍
Product Description
General description
Human UBA6 (UBE1L2) (GenBank Accession No. NM_018227), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
技术参数
Specifications
| biological source | human |
| recombinant | expressed in baculovirus infected insect cells |
| assay | ≥70% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 118 kDa |
| packaging | pkg of 100 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | >0.02 mg/mL |
| NCBI accession no. | NM_018227 |
| UniProt accession no. | A0AVT1 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... UBA6(55236) |
