General description
The pBICEP-CMV-3 bicistronic expression vector is a 5.3 kb derivative of pCMV5 used for transient or stable co-expression of an N-terminal 1´ FLAGÒ fusion protein and a selection gene in mammalian cells.
Application
For transient, cytoplasmic expression of an N-terminal FLAG® fusion and a second gene of interest or selection marker from bicistronic mRNA. Two genes can be cloned into MCS1 and MCS2 for transcription of a single message from the CMV promoter. This vector is useful for protein-protein interaction studies, multi-subunit proteins, and cloning a selection marker of choice.
Biochem/physiol Actions
The presence of two multiple cloning sites not only allows the user to clone a target gene, but a selectable marker of their choice, as well. The promoter-regulatory region of the human cytomegalovirus immediate early promoter drives transcription of the FLAG-fusion construct along with a downstream selection gene. The EMCV IRES region controls translation of the selection gene by recruiting the ribosomal subunits for cap-independent translational initiation. Depending on the mode of selection, stable transfectants can be generated by transfection using the appropriate ESCORTä product line specific for the cell type.
Other Notes
Browse additional application references in our FLAG® Literature portal.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pBICEP-CMV is a trademark of Sigma-Aldrich Co. LLC
| shipped in | dry ice |
| Quality Level | 200 |
| storage temp. | −20°C |
