General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
C-X-C motif chemokine 9 (UniProt: Q07325; also known as Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, HuMIG, MIG, Small-inducible cytokine B9) is encoded by the CXCL9 (also known as CMK, MIG, SCYB9) gene (Gene ID: 4283) in human. CXCL9 is a small cytokine of the CXC chemokine family that lacks the Glu-Leu-Arg (ELR) domain. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form. It is induced in macrophages in response to interferon- and is involved in growth, movement , and activation state of cells that participate in immune and inflammatory responses. It also acts as a chemotactic agent for activated T cells. Its actions are mediated via CXC-chemokine receptor 3 (CXCR3). Overexpression of CXCL9 is reported to reduced tumor progression and metastasis via the inhibition of angiogenesis. CXCL9 is reported to exert its tumor-suppressive function by recruiting tumor-infiltrating lymphocytes in human ovarian cancer. Conversely, CXCL9 can also act directly on tumor cells expressing the CXCR3 receptor to promote cell migration and epithelial mesenchymal transition. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bronger, H., et al. (2016). Br. J. Cancer. 115(5); 553-563).
Immunogen
GST/His-tagged Human recombinant mature CXCL9 (without the signal peptide).
Application
Quality Control Testing
Evaluated by Western Blotting with GST/His-tagged recombinant human CXCL9.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant Human CXCL9.
Tested applications
Affinity Binding Assay: A representative lot of this antibody bound recombinant CXCL9/MIG with a KD of 2.3 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CXCL9/MIG in THP-1 cells treated with IFN- and TNF- (both at 100 ng/mL; 24 h).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with GST/His-tagged recombinant human CXCL9.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant Human CXCL9.
Biochem/physiol Actions
Clone 1H3 is a is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects CXCL9 protein.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1H13, recombinant monoclonal |
| description | 1H13 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 14.02 kDa,observed mol wt ~38 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Unknown |
| Protein ID accession no. | NP_002407.1 |
| UniProt accession no. | Q07325 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CXCL9(4283) |
