General description
Histone PARylation factor 1 (UniProt: Q9NWY4) is encoded by the HPF1 (also known as C4orf27) gene (Gene ID: 54969) in human. Polyadenylate-binding protein 1 (PABP1) is distinguished by its four RNA recognition motifs (RRMs), which are essential for binding to the poly(A) tail of mRNA. It also features a C-terminal domain that facilitates interactions with various proteins, enhancing its role in post-transcriptional regulation of gene expression. PABP1 is crucial in the regulation of mRNA stability and translation. It binds to the poly(A) tails of mRNAs, protecting them from degradation and enhancing their translational efficiency. PABP1 also plays a role in the initiation of translation by interacting with the translation initiation complex and circularizing mRNA to promote reinitiation. PABP1 is ubiquitously expressed across different tissues, reflecting its fundamental role in cellular processes. Its expression may be modulated in response to cellular stress or developmental cues, underlining its adaptiveness in regulatory processes. Alterations in PABP1 function or expression are linked to several pathologies. In cancer, overexpression of PABP1 can lead to the enhanced stability and translation of oncogenic mRNAs, promoting tumor progression. Conversely, reduced PABP1 activity might contribute to neurodegenerative diseases by impairing mRNA stabilization and translation, crucial for neuron function and survival. Additionally, PABP1 is implicated in viral infections, as viruses often exploit it to optimize the translation of viral proteins.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal part of human HPF1.
Application
Quality Control Testing
Evaluated by Western Blotting in Jurkat cell nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HPF1 in Jurkat cell nuclear extract.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HPF1 in Jurkat cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HPF1 in human pancreas tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound HPF1 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1N22 is a ZooMAb® rabbit recombinant monoclonal antibody that detects HPF1. It targets an epitope within 22 amino acids from the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1N22, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 40 kDa,observed mol wt ~42 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgGκ |
| epitope sequence | N-terminal |
| Protein ID accession no. | NP_060337 |
| UniProt accession no. | Q9NWY4 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... HPF1(54969) |
