General description
DNA dC->dU-editing enzyme APOBEC-3B (UniProt: Q9UH17; also known as EC:3.5.4.38, A3B, Phorbolin-1-related protein, Phorbolin-2/3) is encoded by the APOBEC3B gene (Gene ID: 9582) in human. APOBEC3B is a member of the cytidine deaminase gene family. It contains a conserved zinc-binding domain and a C-terminal catalytic domain that is responsible for its deaminase activity. Its pseudo-catalytic N-terminal domain can bind to nucleic acids but does not have deaminase activity. APOBEC3B can cause C-to-U mutations in single-stranded DNA molecules, subsequently inducing hypermutation in the viral genome. APOBEC3B primarily targets retroviruses and retrotransposons. APOBEC3B is expressed in various tissues, including the reproductive organs, lymphoid tissues, and epithelial cells of the respiratory and gastrointestinal tracts. Its expression is up-regulated by - interferon. APOBEC3B has been implicated in the development of several diseases. It has been associated with carcinogenesis, particularly in breast cancer. Elevated expression of APOBEC3B in cancer cells can lead to increased mutagenesis and genomic instability. APOBEC3B-induced mutations contribute to the evolution of tumor genomes and the development of drug resistance. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Brown, WL., et al. (2019). Antibodies (Basel). 8(3); 47, Maiti, A., et al. (2021). Curr. Opin. Struct. Biol. 67; 195-204)
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal region of human DNA dC->dU-editing enzyme APOBEC-3B (APOBEC3B).
Application
Quality Control Testing
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected APOBEC3B in U2OS cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected APOBEC3B in MCF7 and THP-1 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution from a representative lot detected APOBEC3B in Human breast cancer, Human cervical cancer and Human bladder cancer tissue sections.
Enzyme Immunoassay (ELISA) Analysis: A representative lot detected APOBEC3B in ELISA applications (Brown, W., et. al. (2019). Antibodies (Basel). 8(3):47).
Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected APOBEC3B in U2OS cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 5210-87-133 is a ZooMAb® rabbit recombinant monoclonal antibody that detects Human APOBEC3B. It targets an epitope within 28 amino acids from the C-terminal region. May cross-react with human A3A and A3G.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 5210-87-133, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 46 kDa,observed mol wt ~36 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | ELISA: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_004891.4 |
| UniProt accession no. | Q9UH17 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... APOBEC3B(9582) |
