Application
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
Preparation Note
The solution can be used without dialysis (for enzyme immuno-assay).
Other Notes
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C
Sales restrictions may apply
| biological source | bovine (calf) intestinal mucosa |
| form | solution (clear, colorless) |
| specific activity | ~2500 U/mg protein (~10 mg/ml) |
| technique(s) | enzyme immunoassay: suitable |
| impurities | 0.1 mM ZnCl2,1 mM MgCl2,3 M NaCl,30 mM triethanolamine |
| pH | ~7.6 |
| storage temp. | 2-8°C |
