General description
Sphingosine 1-phosphate-induced release of TIMP-2 from vascular smooth muscle cells inhibits angiogenesis.
Application
Sphingosine kinase 1 has been used in a study to assess the modulation of cellular S1P levels. Sphingosine kinase 1 has also been used in a study to investigate how FTY720 normalizes hyperglycemia.
Biochem/physiol Actions
Converts sphingosine to sphingosine-1-phosphate. SPHK1 shares two large conserved regions with SPHK2. While SPHK1 is found only in the cytosol, SPHK2 is localized in both the cytosol and nuclei.
Physical form
Supplied in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween 20, 50% glycerol, 3 mM DTT.
aqueous glycerol solution
Other Notes
One unit will produce 1.0 pmole of sphingosine-1-phosphate from ATP and D-erythro-sphingosine per minute at pH 7.4, 37 °C.
| recombinant | expressed in baculovirus infected insect cells |
| Quality Level | 200 |
| form | solution |
| specific activity | ≥10 units/μg |
| mol wt | 47 kDa |
| UniProt accession no. | Q9NYA1 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... SPHK1(8877) |
