General description
Astrocytic phosphoprotein PEA-15 (UniProt: Q15121; alsoknown as 15 kDa phosphoprotein enriched in astrocytes, Phosphoprotein enrichedin diabetes, PED) is encoded by the PEA15 gene (Gene ID: 8682) in human.PEA-15is a cytoplasmic protein that is ubiquitously expressed. Higher expressions arefound in ear, brain, muscle, and adipose tissue. Higher levels of expressionhave been reported in tissues from individuals with type 2 diabetes. PEA-15blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAPkinase cascade. It is reported to Inhibit RPS6KA3 activities by retaining it inthe cytoplasm. It is also shown to inhibits both TNFRSF6- and TNFRSF1A-mediatedcaspase 8 activity and apoptosis. It also regulates glucose transport by controllingboth the content of SLC2A1 glucose transporters on the plasma membrane and theinsulin-dependent trafficking of SLC2A4 from the cell interior to the surface.PEA-15 is phosphorylated by protein kinase C and calcium-calmodulin-dependentprotein kinase. These phosphorylation events are modulated by neurotransmittersor hormones. PEA-15 has two microtubule binding sites (aa 98-107 and122-129). This ZooMAb® recombinantmonoclonal antibody, generated by our proprietary technology, offerssignificantly enhanced specificity, affinity, reproducibility, and stabilityover conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
GST/His-tagged full-length recombinant human Astrocytic phosphoprotein PEA-15.
Application
Anti-PEA15, clone 2J13 ZooMAb, Cat. No. ZRB1444, is a recombinant Rabbit monoclonal antibody that specifically targets PEA15 and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PEA15 in NIH3T3 and C6 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PEA15 in NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PEA15 in human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant PEA15 protein with a KD of 2.7 x 10-5 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 2J13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Astrocytic phosphoprotein PEA-15.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2J13, monoclonal,recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 15.04 kDa,observed mol wt 15 kDa |
| species reactivity | rat, mouse, human, monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | Q15121 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PEA15(8682) |
