General description
The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.
Application
HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select® Cobalt Affinity Gel has been used to purify His-tagged proteins.
Physical form
1:1 suspension in a 30% ethanol solution
Legal Information
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
| Quality Level | 200 |
| form | (1:1 suspension in a 30% ethanol solution) |
| shelf life | 1 yr (when stored properly.) |
| technique(s) | protein purification: suitable |
| matrix | 6% Beaded Agarose |
| capacity | >15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein) |
| storage temp. | 2-8°C |
