产品介绍
Product Description
Application
Functional Genomics/Screening/Target Validation
- Positive control, HPRT1, CRISPR lentivirus DNA format, for creating gene knockouts/genetic modifications in cell lines.
To see more application data, protocols, vector maps visit Sigma.com/crispr.
Features and Benefits
Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPR DNA. The number of cells with HPRT1 knocked out increases over time with 6−TG selection.
Physical form
The Sigma CRISPR Lenti Human HPRT1 Positive Control Vector is provided as 1 μg of plasmid DNA in Tris−EDTA (TE).
Legal Information
CRISPR Use License Agreement
Lentiviral and WPRE License Agreements
技术参数
Specifications
| packaging | vial of 50 μL |
| Quality Level | 200 |
| concentration | 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) |
| application(s) | CRISPR |
| shipped in | dry ice |
| storage temp. | −20°C |
