General description
Anti-Monkeypox virus /MPXV A35R Antibody, clone SAA0287 is a monoclonal antibody produced in mouse. It is a single, specific antibody produced from a single clone of B cells. It is composed of two heavy chains and two light chains linked by disulfide bonds. The two heavy chains are linked to each other by non-covalent bonds and the light chains are linked to the heavy chains by disulfide bonds.
Monkeypox virus (MPXV), like smallpox virus, belongs to the genus Orthopoxvirus. Its morphology is consistent with that of orthopoxvirus, with a brick-shaped or oval shape with rounded corners and a size of 200~400nm. Brick-shaped or oval-shaped, covered by a lipoprotein membrane or envelope, with two protein-containing side bodies in the middle, and a thick membrane core containing a large double-stranded DNA gene body. The genome of Mpox virus is a double-stranded DNA, about 197kb long, and the end of the genome contains an identical but opposite terminal inverted repeat sequence. The virus contains 190 open reading frames, 4 of which are located in terminal inverted repeats. The core proteins of monkeypox virus are A29L, A35R, B6R, M1R, H3L.
Application
ELISA,neutralization
Biochem/physiol Actions
Monoclonal Anti-Monkeypox virus /MPXV A35R Antibody, clone SAA0287 binds to the viral antigen, specifically the A35R protein of the Monkeypox virus. This binding inhibits the virus from entering the host cell and prevents the virus from replicating.
Packaging
100µg per vial / 1 mg per vial
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.
| biological source | mouse |
| Quality Level | 100 |
| recombinant | expressed in CHO cells |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| clone | SAA0287, recombinant monoclonal |
| assay | >95% (SDS-PAGE) |
| form | liquid |
| mol wt | 150 kDa |
| species reactivity | monkeypox virus |
| concentration | 1 mg/mL |
| technique(s) | ELISA: 1:2000-1:4000,neutralization: 0.01-10 μg/mL |
| color | colorless |
| pH | 7.4 |
| isotype | IgG2a, kappa |
| Protein ID accession no. | NP_536572.1 |
| UniProt accession no. | Q80KX2,Q8V4U4 |
| shipped in | wet ice |
| storage temp. | -65 to -96°C |
| target post-translational modification | unmodified |
