General description
The rat monoclonal antibody GL-1 reacts with CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Immunogen
LPS-activated CBA/Cs mouse splenic B cells
Application
The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 2 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Features and Benefits
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Physical form
Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.
| biological source | rat |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | GL-1, monoclonal |
| form | buffered aqueous solution |
| species reactivity | mouse |
| concentration | 1 mg/mL |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2a |
| NCBI accession no. | NM_019388.3 |
| UniProt accession no. | P42082 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Cd86(12524) |

