General description
Gelsolin is an actin-severing protein found in mammalian cells and blood plasma. Has tumor suppressor activity in several cancer cell lines and in nude mice.
Monoclonal Anti-Gelsolin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes of an immunized mouse. Gelsolin (GSN) is encoded by the gene mapped to human chromosome 9q33.2. GSN is a calcium dependent actin binding protein. It has been shown to exist in at least two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin has also been called as brevin. GSN is characterized with six domains G1-G6 at N-terminal end, each domain contains Ca2+ binding site.
Immunogen
human plasma gelsolin.
Application
Monoclonal Anti-Gelsolin antibody produced in mouse has been used in immunoprecipitation and immunohistochemical staining.
Biochem/physiol Actions
Gelsolin (GSN) is a potent modulator of actin filament length and gelation. It binds to the plus′ ends of actin monomers and filaments, preventing monomer exchange. GSN plays a vital role in regulation of actin dynamics. Mutations in this gene cause familial amyloidosis Finnish type (FAF) characterized by cranial neuropathy and lattice corneal dystrophy. Plasma gelsolin is used as a biochemical marker for acute rheumatic carditis.
The antibody is specific for an epitope located on the 47 kDa peptide derived from a chymotryptic cleavage of gelsolin. The antibody reacts with plasma and cytoplasmic gelsolin. An additional application for this antibody is immunoaffinity chromatography, for purification of gelsolin, and for detection of gelsolin-actin complexes in cell extracts.
Other Notes
This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200750 Anti-Gelsolin antibody, Mouse Monoclonal
clone GS-2C4, purified from hybridoma cell culture
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | GS-2C4, monoclonal |
| contains | 15 mM sodium azide |
| species reactivity | bovine, pig, human, rabbit |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using heat-induced antigen retrieval sections,immunohistochemistry (frozen sections): suitable,indirect ELISA: suitable,microarray: suitable,western blot: 1:1,000 using human plasma |
| isotype | IgG1 |
| UniProt accession no. | P06396 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... GSN(2934) |
