General description
Chromobox protein homolog 5 (UniProt: P45973; also known as Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha) is encoded by the CBX5 (also known as HP1A) gene (gene ID: 23468) in human. Chromobox protein homolog 5 (CBX5) is a component of heterochromatin that recognizes and binds histone H3 tails methylated at lysine 9 (H3K9me), leading to epigenetic repression. It is excluded from chromatin when tyrosine 41 of histone H3 is phosphorylated (H3Y41ph) by JAK2. It can also interact with lamin-B receptor (LBR) and this interaction can contribute to the association of the heterochromatin with the inner NuCLEAR™ membrane. It has a single N-terminal Chromo domain (aa 20-78) that can bind to histone proteins via methylated lysine residues, and a C-terminal Chromo shadow-domain (aa 121-179), which is responsible for the homodimerization and interaction with a number of chromatin-associated non-histone proteins. CBX5 is a suppressor of metastasis, and it is down-regulated at the transcriptional and protein level in metastatic compared to non-metastatic breast cancers. In breast cancer, its expression level correlates with both clinical outcome in terms of patient survival and clinical data in terms of tumor size and stage of this disease. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity™, reproducibility, and stability over conventional monoclonals. (Ref.: Vad-Nielsen, J., et al. (2016). BMC Cancer 16; 32; Dawson, MA., et al. (2009). Nature. 461(7265); 819-822).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Chromobox protein homolog 5 (CBX5).
Application
Quality Control Testing
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CBX5 in A431 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CBX5 in U2OS cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CBX5 in Human gallbladder tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CBX5 in U2OS cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 5I2-K is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Chromobox protein homolog 5 (CBX5). It targets and epitope within 15 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
Affinity is a trademark of Mine Safety Appliances Co.
NuCLEAR is a trademark of Sigma-Aldrich Co. LLC
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 5I2-K, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey, rat, mouse |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_001120793 |
| UniProt accession no. | P45973 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
