General description
Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.
Immunogen
KLH标记的含有水泡性口炎病毒糖蛋白（VSV-G）的15个羧基末端氨基酸（497-511）的合成肽。
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.
Biochem/physiol Actions
该抗体可识别包含VSV糖蛋白的五个羧基末端氨基酸的表位。在受感染的细胞中，该抗体将未成熟型VSV-G定位在粗糙内质网（RER）和高尔基复合体潴泡中，将成熟型VSV-G定位在细胞表面和出芽病毒中。该抗体不会染色缺少膜和细胞质结构域的分泌型VSV-G。该抗体可用于微注射抗体、免疫印迹、免疫沉淀、免疫细胞化学和免疫电镜法研究新合成的VSV-G在向质膜和细胞表面转移过程中细胞质结构域的作用。该抗体可用于对以VSV-G羧基末端标记的外源性导入结构进行检测、免疫沉淀和免疫细胞化学染色。该标记不会干扰所研究蛋白质的功能，可被P5D4抗体特异性识别，而不与任何内源性蛋白质发生交叉反应。
Physical form
Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Preparation Note
Prepared using cyanogen bromide-activated agarose.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | agarose conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | P5D4, monoclonal |
| form | PBS suspension |
| analyte chemical class(es) | proteins (VSV-G) |
| technique(s) | immunoprecipitation (IP): suitable,protein purification: suitable |
| isotype | IgG1 |
| capacity | ≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein) |
| shipped in | wet ice |
| storage temp. | 2-8°C |
