General description
K2P1.1 (also named TWIK-1 or KCNK1) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 14 members. These channels show little time or voltage dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents, which help set the membrane resting potential and cell excitation.
The gene K2P1.1 (two pore domain K+ channel), also referred to as KCNK1 (potassium two pore domain channel subfamily K member 1), encodes a protein that spans a length of 337-amino acids. It contains four transmembrane regions and the characteristic sequence of potassium channels in the two pore regions. The protein is found to be exclusively expressed in distal tubule. The gene is mapped to human chromosome 1q42.2.
Immunogen
synthetic peptide RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human K2P1.1 (KCNK1).
Biochem/physiol Actions
The gene KCNK1 (potassium two pore domain channel subfamily K member 1) is a double-pore potassium channel that functions in maintaining the renal potassium homeostasis. KCNK channel proteins are involved in cell volume, apoptosis, and proliferation. Alterations in these proteins have been associated with various diseases. Decreased levels of KCNK1 in human cardiac atria have been implicated in chronic atrial fibrillation. It functions as a suppressor of anchorage-independent growth in fibroblast.
Physical form
Lyophilized from pphosphate buffered saline, pH 7.4, containing 1% BSA and 0.025% sodium azide.
Preparation Note
Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | lyophilized powder |
| species reactivity | mouse, human, rat |
| technique(s) | immunohistochemistry: suitable,western blot: 1:200 using rat brain lysates,western blot: 1:300 using HEK-K2P1.1 transfected cells |
| UniProt accession no. | O00180 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... KCNK1(3775) mouse ... Kcnk1(16525) rat ... Kcnk1(59324) |
