General description
CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.
Immunogen
MOLT-4 (human T-ALL cell line)
Biochem/physiol Actions
The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
Physical form
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | APC-Cy7 conjugate |
| Quality Level | 100 |
| antibody product type | primary antibodies |
| clone | M38, monoclonal |
| form | buffered aqueous solution |
| species reactivity | ( Human), rabbit, ( Cat) |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1 |
| UniProt accession no. | P60033 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CD81(975) |
