General description
A cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC50 = 2.4 µM; Kd = 16 nM) and 373 (IC50 = 5.13 µM Kd = 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC50 = 125 µM; Kd = 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).
Please note that the molecular weight for this compound is batch-specific due to variable water content.
Biochem/physiol Actions
Primary Target
miRNA-272/273
Reversible: yes
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-2-°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Vo, D.D., et al. 2014. ACS Chem. Biol.9, 711.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| assay | ≥98% (HPLC) |
| Quality Level | 100 |
| form | solid |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,desiccated (hygroscopic),protect from light |
| color | off-white |
| solubility | DMSO: 100 mg/mL |
| storage temp. | −70°C |
