General description
The antibody reacts specifically against insulin by RIA and immunocytochemistry. It exhibits cross-reactivity with human proinsulin. The antibody binds to insulin with an affinity constant of 8.8 × 109 M-1 in RIA. The insulin gene is mapped to human chromosome 11p15.5.
Human insulin is made up of 51 amino acids. It consists of two peptide chains, 21 amino acids make up A-chain and chain B contains 31 amino acids. The two chains are linked together by a disulfide bond, to form a classical insulin fold with three α helices, chain A having two and one in chain B.
Application
请参考Protocol了解详情。
Biochem/physiol Actions
Abnormal insulin secretion or function, leads to diabetes mellitus, obesity and other carbohydrate metabolism disorders. Insulin is known to stimulate cell division. The biological effects of insulin are mediated by insulin receptor and it integrates major signaling pathways.
Other Notes
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| species reactivity | porcine |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable |
| input | sample type plasma sample type cell culture supernatant(s) sample type serum |
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 4 μIU/mL standard curve range: 4.69 μIU/ml-300 μIU/ml |
| detection method | colorimetric |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | Porcine ... INS(397415) |
