General description
Interleukin-1α (IL-1α) is a cytokine that belongs to the interleukin-1 (IL-1) family. It is located intracellularly in hematopoietic and non-hematopoietic cells. IL-1α gene is located on the human chromosome at 2q14.1.
Application
Interleukin-1α (IL-1α) has been used:
- to study its effects on human colorectal adenocarcinoma cells
- to stimulate primary human fetal retinal pigment epithelium (hfRPE) cells
- to pre-stimulate human THP-1 monocytes
Biochem/physiol Actions
Interleukin-1α (IL-1α) plays a crucial role in inflammation during cell necrosis. It also regulates the recruitment of interleukin-1β (IL-1β), neutrophils, chemokines, and other cytokines at the site of injury. IL-1α exhibits a dual function of binding to its cell surface receptor as well as chromatin.
Physical form
Lyophilized from 0.2 μm filtered 10 mM sodium phosphate , pH 8.0, plus 50 mm NaCl and 50 μg bovine serum albumin (BSA) per μg cytokine.
Analysis Note
The proliferative activity of human IL-1α is tested in culture using the murine cell line D10S.
| biological source | human |
| Quality Level | 100 |
| recombinant | expressed in E. coli |
| assay | ≥98% (SDS-PAGE and HPLC) |
| form | lyophilized powder |
| quality | endotoxin tested |
| mol wt | ~17 kDa |
| packaging | pkg of 2 and 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01583 |
| storage temp. | −20°C |
| Gene Information | human ... IL1A(3552) |
