General description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Immunogen
A synthetic acetylated peptide corresponding to residues surrounding K18 of human H3
Physical form
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Preparation Note
Store at -20℃. Avoid freeze / thaw cycles.
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | 15 |
| species reactivity | rat, mouse, human |
| concentration | 0.57 mg/ml |
| technique(s) | ChIP: 1:50-1:100,immunofluorescence: 1:500-1:1000,immunohistochemistry: 1:200-1:500,immunoprecipitation (IP): 1:200-1:500,western blot: 1:1000-1:3000 |
| UniProt accession no. | P68431 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | acetylation (Lys18) |
| Gene Information | human ... H3C12(8356) |
