General description
Contains amino acid residues [77-436] of HIV-1 gag antigen.
The human immunodeficiency virus 1 (HIV−1) gag antigen is a recombinant 39 kDa protein encoded by the GAG gene of the human immunodeficiency virus (HIV) type I expressed in and isolated from Escherichia coli. It has a β−galactosidase tag (114 kDa) on the N−terminus. HIV-1 Gag exists as monomers or lower-order oligomers in the cytosol. At the plasma membrane, it forms higher-order multimers.
Application
HIV-1 gag has been used in the coculture with an autologous cluster of differentiation T (CD4+ T) to study the ability of monocyte-derived dendritic cells (MDC1) to induce human immunodeficiency virus 1 (HIV−1) latency reversal (LR).
Biochem/physiol Actions
Positive control for HIV antibodies.
The human immunodeficiency virus 1 (HIV−1) gag (p24) antigen reacts strongly with HIV positive serum and may be used as a positive control for evaluating antibodies to HIV. At transcription sites, HIV-1 Gag creates ribonucleoprotein complexes with unspliced viral RNA. The HIV-1 Gag protein is accountable for packaging genomic RNA (gRNA) and the assembly of immature viral particles.
Physical form
Solution in 8 M urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.
| recombinant | expressed in E. coli |
| Quality Level | 200 |
| assay | ≥95% (SDS-PAGE) |
| mol wt | 39 kDa (plus 114 kDa β-Gal tag) |
| concentration | 1 mg/mL |
| UniProt accession no. | P04591 |
| storage temp. | −20°C |
| Gene Information | human immunodeficiency virus 1 ... gag(155030) |
