Biochem/physiol Actions
Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.
| biological source | human |
| Quality Level | 200 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| assay | ≥97% (SDS-PAGE and N-terminal analysis) |
| form | lyophilized powder |
| potency | 0.01-0.05 ng/mL ED50 |
| mol wt | glycoprotein 75 kDa |
| packaging | pkg of 5 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | P29460">P29459, P29460 |
| storage temp. | −20°C |
| Gene Information | human ... IL12A(3592), IL12B(3593) |
