Application
RANK is the essential signaling receptor for osteoclast differentiation factor in osteoclastogenesis. The biological functions mediated by RANK include activation of NF-
Biochem/physiol Actions
TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand, osteoprotegerin ligand and osteoclast differentiation factor) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-κB and c-jun N-terminal kinases, enhancement of T cell growth and dentritic cell function, induction of osteoclastogenesis, and lymph node genesis. Soluble RANK is able to block TRANCE-induced biological activity.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
Analysis Note
The biological activity is measured by its ability to inhibit TRANCE-induced osteoclast differentiation in mouse splenocytes.
| biological source | mouse |
| Quality Level | 200 |
| recombinant | expressed in NSO cells |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 10.12 μg/mL EC50 |
| mol wt | 60 kDa |
| packaging | pkg of 1 mg |
| storage condition | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | O35305 |
| storage temp. | −20°C |
| Gene Information | mouse ... Tnfrsf11a(21934) |
