General description
The serum- and glucocorticoid-regulated kinase (SGK1) is a member of the AGC kinase family and the gene encoding it is localized on human chromosome 6q23.
Biochem/physiol Actions
The serum- and glucocorticoid-regulated kinase (SGK1) has a role in the hormonal regulation of ion channels and pumps. It has been associated with cancers. The protein modulates nitric oxide production by activating NO synthase. SGK1 protects cells from apoptosis and oxidative stress.
Kinase assays.
Physical form
Solution in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 0.15 M NaCl, 0.03% Brij® L23, 1 mM benzamidine, 0.2 mM PMSF. and 270 mM sucrose.
Other Notes
One unit of SGK1 = 1 nmol phosphate incorporated into crosstide substrate peptide in one minute.
Legal Information
Brij is a registered trademark of Croda International PLC
| recombinant | expressed in baculovirus infected Sf9 cells (truncated human sequence (a.a. 1-60 deleted, S422D mutation)-histidine-tagged) |
| Quality Level | 200 |
| form | buffered aqueous solution |
| mol wt | ~48 kDa by SDS-PAGE |
| UniProt accession no. | O00141 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... SGK1(6446) |
