General description
C-C motif chemokine receptor 7 (CCR7) also known as EBI1, is encoded by the gene mapped to human chromosome 17q12-q21.2. The encoded protein is a member of chemokine receptor family and is expressed mainly by B cells, T cells, and on activated mature dendritic cells (DCs).
CCR7 is a G protein-coupled seven-transmembrane domain receptor. It has two high-affinity ligands, secondary lymphoid chemokine (SLC) and EB11-ligand chemokine (ELC).
Immunogen
The antiserum was produced against synthesized peptide derived from human CCR7.
Immunogen Range: 170-219
Application
Anti-CCR7 antibody produced in rabbit has been used for proximity ligation assay (PLA).
Anti-CCR7 antibody produced in rabbit has been used in immunofluorescence (1:50).
Biochem/physiol Actions
C-C motif chemokine receptor 7 (CCR7) harmonizes the antigen-specific immune response by combining T cells, B cells, and dendritic cells (DCs), to form functional microenvironments in the secondary lymphoid organs. CCR7 controls trafficking of skin DC under both inflammatory and steady-state conditions. This protein is also involved in emigration pathway of mature DCs from the skin to regional lymph nodes in vivo. CCR7 oligomers function acts as a scaffolds to link distinct signaling pathways for cell migration. Interaction of CCR7 with its ligand, CCL21/6ckine is essential for preferential lymph node metastasis of gastric carcinoma.
CCR7 can stimulate the development of hepatocellular carcinoma (HCC). In human malignancies, CCR7 may act as an initiator of epithelial-mesenchymal transition (EMT).
Features and Benefits
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Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen 42 kDa |
| species reactivity | human |
| concentration | ~1 mg/mL |
| technique(s) | ELISA: 1:40000,immunofluorescence: 1:100-1:500,western blot: 1:500-1:1000 |
| NCBI accession no. | P32248 |
| UniProt accession no. | P32248 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CCR7(1236) |
