General description
Interleukin-4 (UniProt: P05112; also known as B-cell stimulatory factor 1 (BSF-1), Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra) is encoded by the IL4 gene (Gene ID: 3565) in human. Interleukin-4 is a pleiotropic cytokine with a well-defined helical bundle structure. It plays a crucial role in regulating immune responses, particularly in promoting the differentiation of naive T cells into Th2 cells and stimulating B cell growth and antibody production. Interleukin-4 is primarily produced by activated T cells, basophils, and mast cells, and its expression can be induced by various stimuli, including antigens, T cell receptor signaling, and cytokines such as IL-2 and IL-4 itself. Upon secretion, Interleukin-4 binds to its receptor, initiating signaling cascades that activate the JAK-STAT pathway and modulate gene expression. Dysregulation of Interleukin-4 has been linked to allergic diseases, such as asthma and atopic dermatitis, as well as autoimmune disorders and certain cancers, highlighting its significance as a therapeutic target in these conditions. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal part of human Interleukin-4.
Application
Quality Control Testing
Evaluated by Western Blotting with human recombinant Interleukin-4.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected human recombinant Interleukin-4 .
Tested Applications
Affinity Binding Assay Analysis: The binding affinity of this antibody to Interleukin-4 peptide was at least one thousand-fold (1,000X) higher than that obtained with the non-specific control peptide.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Interleukin-4 in MOLT-4 cells with and without treatment with TPA (40 nM, 6 hrs) and Ionomycin (2 uM 6 hrs) and then treated with Brefeldin A (1ug/mL) for the last 5 .
Enzyme Immunoassay (ELISA): A representative lot of this antibody detected Interleukin-4 protein in ELISA application.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2C14 is a ZooMAb® rabbit recombinant monoclonal antibody that detects Interleukin-4. It targets an epitope within 25 amino acids from the C-terminal part.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2C14, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 17.49 kDa,observed mol wt ~16 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgGκ |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_000580 |
| UniProt accession no. | P05112 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... IL4(3565) |
