General description
KAT4 is a DNA-binding protein complex required for RNA polymerase II-mediated transcription of many, if not all, protein-encoding genes in eukaryotic cells. KAT4 plays a key role in the initiation process, since it binds to the TATA element to form a complex that nucleates the assembly of the other components into a preinitiation complex and that may be stable through multiple rounds of transcription. KAT4 may be targeted to specific chromatin-bound promoters and may play a key role in chromatin recognition. KAT4 also serves to link the control of transcription to the cell cycle.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~155 kDa |
| NCBI accession no. | NM_138923 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... TAF1(6872) |
