General description
Non-POU Domain-Containing Octamer-Binding Protein (NONO) is ubiquitously expressed and is composed of two tandem RNP-type RNA-recognition motifs and a putative helix-turn-helix domain followed by a highly charged region. It is enriched in paraspeckles, a nucleoplasmic compartment, and relocalizes to cap structures at the nucleolar periphery when transcription is inhibited.
Non-POU domain containing octamer binding protein (NONO) is part of the Drosophila behavior/human splicing (DBHS) protein family. It has a molecular weight of 54kDa and the gene encoding it is localized on human chromosome Xq13.1.
Application
Anti-NONO (N-terminal) antibody produced in rabbit has been used in immunoprecipitation, immunoblotting. It may be used for immunofluorescence.
Biochem/physiol Actions
Non-POU Domain-Containing Octamer-Binding Protein (NONO) is a nuclear protein implicated in numerous processes including transcription, pre-mRNA processing, nuclear retention of edited RNA, and DNA relaxation. NONO is phosphorylated on multiple sites during mitosis and is a target of the peptidylprolyl isomerase (Pin1).
Non-POU domain containing octamer binding protein (NONO) modulates transcription. Loss-of-function of the protein has been linked to human intellectual disability. The protein also has a role in synaptic transcription and regulation of the circadian clock. NONO also has a function in DNA damage response.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~55 kDa |
| species reactivity | human |
| concentration | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates,indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells,western blot: 1-2 μg/mL using NIH-3T3 lysate |
| UniProt accession no. | Q15233 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... NONO(4841) mouse ... Nono(53610) rat ... Nono(317259) |
