产品介绍
Product Description
General description
Sigma的杂交液是一种2倍即用型杂交缓冲液，适用于Northern和Southern印迹方案。
Application
杂交液已用于硝酸纤维素膜的杂交。它也已用于DNA杂交。
适合用作Northern和Southern印迹方案的杂交缓冲液。
Preparation Note
Sigma的杂交液含200 μg/mL DNA，溶剂为10倍SSC和2倍Denhardt溶液：
200 μg/mL DNA
2mg牛血清白蛋白(BSA)
2mg Ficoll
2mg聚乙烯吡咯烷酮(PVP)
0.15 M柠檬酸钠
1.5 M NaCl
技术参数
Specifications
| Quality Level | 100 |
| form | liquid (2x concentrate) |
| foreign activity | DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected |
| storage temp. | −20°C |
