General description
Anti-p27 KIP1 Antibody detects endogenous levels of total p27 KIP1 protein.
Cyclin-dependent kinase inhibitor 1B (CDKN1B or p27 KIP1) is a member of CDK inhibitor (CKI). The CDKN1B gene is mapped to human chromosome 12p13.1. The prime domains in CDKN1B include the nuclear export signal (NES) and the nuclear localization signal (NLS) and the CDK binding domain.
Immunogen
The antiserum was produced against synthesized peptide derived from human p27 Kip1.
Immunogen Range: 1-50
Application
Anti-p27 KIP1 antibody produced in rabbit has been used in western blotting (1:500).
Biochem/physiol Actions
Cyclin-dependent kinase inhibitor 1B (CDKN1B) negatively regulates cell cycle events by binding to cyclin-dependent kinase. It is a tumor suppressor gene correlated with the pathophysiology of tumors. Germline mutations in CDKN1B are implicated in multiple endocrine neoplasia (MEN) syndrome and MEN-like syndrome (MENX). It is a potent biomarker in acute myeloid leukemia.
Features and Benefits
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Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen 22 kDa |
| species reactivity | rat, mouse, human |
| concentration | ~1 mg/mL |
| technique(s) | ELISA: 1:10000,immunohistochemistry: 1:50-1:100,western blot: 1:500-1:1000 |
| NCBI accession no. | P46527 |
| UniProt accession no. | P46527 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CDKN1B(1027) |
