General description
ATP citrate lyase (ACLY) is encoded by the gene mapped to human chromosome 17q21.2. It is localized in the cytoplasm and is widely expressed in mammalian tissues.. ACLY is a 440 KDa tetrameric protein containing four apparently identical subunits.
Immunogen
The antiserum was produced against synthesized peptide derived from human ATP-Citrate Lyase around the phosphorylation site of Ser454.
Immunogen Range: 420-469
Application
Anti-phospho-ATP-Citrate Lyase (pSer454) antibody produced in rabbit has been used in immunohistochemistry.
Biochem/physiol Actions
ACLY (ATP citrate lyase) is responsible for cleavage of mitochondria-derived citrate to generate oxaloacetic acid (OAA) and acetyl-CoA, which is required for lipid biogenesis. This enzyme couples energy metabolism with fatty acids and facilitates cell growth. In addition, ACLY is also essential for DNA-damage-induced histone acetylation. ACLY is upregulated in several types of cancers and is associated with cancer cell proliferation. Elevated expression of the gene leads to the development of breast cancer. Thus, ACLY -related inhibitors can be considered as a potential strategy for treating breast cancer.
Features and Benefits
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Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen 120 kDa |
| species reactivity | mouse, rat, human |
| concentration | ~1 mg/mL |
| technique(s) | ELISA: 1:1000,immunohistochemistry: 1:50-1:100,western blot: 1:500-1:1000 |
| NCBI accession no. | P53396 |
| UniProt accession no. | P53396 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer454) |
| Gene Information | human ... ACLY(47) |
