产品介绍
Product Description
Application
Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.
Biochem/physiol Actions
Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome.
Physical form
Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.
Other Notes
One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP
技术参数
Specifications
| recombinant | expressed in E. coli |
| Quality Level | 200 |
| assay | ≥85% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| enzyme activity | ≥10 units/mg protein |
| mol wt | protein apparent mol wt ~80 kDa |
| UniProt accession no. | P51955 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... NEK2(4751) |
