产品介绍
Product Description
General description
Human PRMT6 (GenBank Accession No. NM_018137), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 35 kDa, expressed in FreeStyle 293-F cells.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml FLAG peptide, 10% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Legal Information
FreeStyle is a trademark of Invitrogen Corp.
技术参数
Specifications
| biological source | human |
| recombinant | expressed in FreeStyle™ 293-F cells |
| assay | ≥70% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 35 kDa |
| packaging | pkg of 20 μg |
| manufacturer/tradename | Invitrogen |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | >0.02 mg/mL |
| NCBI accession no. | NM_018137 |
| UniProt accession no. | Q96LA8 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PRMT6(55170) |
