General description
Monoclonal Anti-Cytokeratin, pan (mouse IgG1 isotype) is derived from the C-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431. Cytokeratin is a member of the intermediate filaments family.
Application
Monoclonal Anti-Cytokeratin pan antibody has been used in immunoblotting, dot blot, immunohistochemistry, immunocytochemistry, immunofluorescence, immunostaining, detecting micro-metastases in lymph nodes, bone marrow, and other tissues and for determining the origin of poorly differentiated tumors.
Biochem/physiol Actions
Cytokeratin plays a major role in observing carcinomas.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | C-11, monoclonal |
| form | buffered aqueous solution |
| technique(s) | immunocytochemistry: 15-30 μg/mL using methanol/acetone-fixed PTK-2 cells or A549 - human lung carcinoma cells,immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15-30 μg/mL using human skin,immunohistochemistry (frozen sections): suitable,microarray: suitable,western blot: suitable |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
