产品介绍
Product Description
Biochem/physiol Actions
MEKK2 (also known as mitogen-activated protein kinase (MAPK) kinase kinase 2) is a major upstream activator of the JNK/MAPK cascade. MEKK2 can mediate in vitro phosphorylation of MKK4 and MKK7 which are key MAPKs that activate JNK. In addition, MEKK2 immunoprecipitates activated JUN in an IL1-dependent manner. Rheumatoid arthritis and osteoarthritis synovial tissues show high levels of MEKK2 indicating that it may be an important activator of the JNK pathway in arthritis. Endogenous MEKK2 is localized mainly in the cytosol of unstimulated cells.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
技术参数
Specifications
| recombinant | expressed in baculovirus infected Sf9 cells |
| Quality Level | 200 |
| product line | PRECISIO® Kinase |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| specific activity | 192-260 nmol/min·mg |
| mol wt | ~115 kDa |
| UniProt accession no. | Q9Y2U5 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... MAP3K2(10746) |
