General description
IL-34 is a ligand for colony-stimulating factor-1 receptor (CSF1R), which also binds to CSF-1. It specifically binds CD14+ monocytes, promotes survival and proliferation of human peripheral blood monocytes, and stimulates macrophage colony formation by human bone marrow cells. IL-34, like human CSF-1 stimulates phosphorylation of MAPK1/ERK2 and MAPK3/ERK1.
Biochem/physiol Actions
IL-34 is a homodimeric cytokine that is expressed in a range of tissues including spleen, heart, brain, liver, kidney, lung, ovary, thymus, testis, small intestine, prostate and colon. Recombinant human IL-34 is a 52.5 kDa homodimeric glycoprotein consisting of 460 amino acid residues, including a C-terminal His-Tag.
Physical form
Lyophilized from 20 mM Sodium Citrate, pH 3.0 + 75 mM NaCl.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
| biological source | human |
| recombinant | expressed in HEK 293 cells |
| assay | ≥95% (HPLC),≥95% (SDS-PAGE) |
| form | lyophilized |
| mol wt | 52.5 kDa |
| packaging | pkg of 10 μg |
| impurities | <0.1 EU/μg endotoxin, tested |
| UniProt accession no. | Q6ZMJ4 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... IL34(146433) |
