General description
Anti-Monkeypox virus/MPXV E8L Antibody, clone SAA0285, is a hybrid protein of mouse and human origin, consisting of a single light chain and a single heavy chain. The light chain is composed of a variable region (VL) and a constant region (CL) while the heavy chain is composed of a variable region (VH) and a constant region (CH). The VL and VH regions are responsible for the binding of the antibody to the antigens on the Monkeypox virus, while the CL and CH regions are responsible for the production of anti-viral proteins.
Monkeypox virus (MPXV), like smallpox virus, belongs to the genus Orthopoxvirus. Its morphology is consistent with that of orthopoxvirus, with a brick-shaped or oval shape with rounded corners and a size of 200~400nm. Brick-shaped or oval-shaped, covered by a lipoprotein membrane or envelope, with two protein-containing side bodies in the middle, and a thick membrane core containing a large double-stranded DNA gene body. The genome of Mpox virus is a double-stranded DNA, about 197kb long, and the end of the genome contains an identical but opposite terminal inverted repeat sequence. The virus contains 190 open reading frames, 4 of which are located in terminal inverted repeats. The core proteins of monkeypox virus are A29L, A35R, B6R, M1R, H3L.
Application
ELISA,neutralization
Biochem/physiol Actions
Monoclonal Anti-Monkeypox virus/MPXV E8L Antibody, clone SAA0285 binds to the antigens on the surface of the Monkeypox virus, blocking the virus from entering host cells. It also activates the production of anti-viral proteins, which neutralize the virus and inhibit its replication. The antibody also activates the immune system, helping to clear the virus from the body.
Packaging
100µg per vial / 1 mg per vial
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.
| biological source | mouse |
| Quality Level | 100 |
| recombinant | expressed in CHO cells |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| clone | SAA0285, recombinant monoclonal |
| assay | >95% (SDS-PAGE) |
| form | liquid |
| mol wt | 150 kDa |
| species reactivity | monkeypox virus |
| concentration | 1 mg/mL |
| technique(s) | ELISA: 1:2000-1:4000,neutralization: 0.01-10 μg/mL |
| color | colorless |
| pH | 7.4 |
| isotype | IgG2a, kappa |
| Protein ID accession no. | NP_536532.1 |
| UniProt accession no. | Q8V4Y0 |
| shipped in | wet ice |
| storage temp. | -65 to -96°C |
| target post-translational modification | unmodified |
