Anti-STOM antibody produced in rabbit，100UL，HPA010961-100UL，Sigma

General description

STOM (stomatin) forms a model for a superfamily of proteins which includes stomatin, the stomatin-like proteins, podocin, flotillins, prohibitins and HFLK which is a bacterial protein. It was initially identified in erythrocytes with abnormal cation permeability. This protein is made of 287 amino acids, has a highly charged N-terminus composed of 24 amino acids, a transmembrane domain of 29 amino acids, and a C-terminus which is made of 234 residues. It has similar topology to caveolin, where its hydrophobic transmembrane region forms a hairpin loop and the N- and C-termini face the cytoplasm. STOM is a lipid raft associated protein which has a molecular weight of 32kDa. In epithelial cell membrane, STOM organizes into homooligomers with a molecular weight of 300kDa. In erythrocyte membrane, the weight of these oligomers ranges from 200 to 600kDa. This gene is located on human chromosome 9q34.1.

Immunogen

Erythrocyte band 7 integral membrane protein recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

STOM (stomatin) is involved in the permeability of erythrocytes to Na⁺/K⁺. Its deficiency leads to hereditary stomatocytosis or cryohydrocytosis, which is characterized by abnormal cell volume due to increased permeability to Na⁺/K⁺. Thus, this protein might also act as a part of ion channel. In erythrocytes, STOM is bound to the actin cytoskeleton and regulates Ca²⁺-induced vesicle formation. This protein might act as a scaffold for certain lipid rafts, which might interact with other proteins to form large protein-lipid structures. These structures might be involved in transport and signaling. Studies suggest that this protein might be involved in the development of definitive red blood cells. STOM protein associates with lipid bodies (LB) under certain conditions. As LBs are metabolically active, STOM might be involved in the regulation of metabolism.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Other Notes

Corresponding Antigen APREST72058

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.