General description
This phosphatase Inhibitor Cocktail contains inhibitors with a broad specificity for L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases. Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.
This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® P0001 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P0044.
Features and Benefits
- Does not freeze in storage for quick, convenient use.
- Specific for Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase.
- Suggested as 100 X solution.
- Reclosable screw-top vials.
- Can be stored in cooler for approx. 1 month or room temperature for a week.
Preparation Note
This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany
| Quality Level | 200 |
| form | ready-to-use solution |
| packaging | pkg of 1 mL (in amber polypropylene vial),pkg of 5 mL (in amber polypropylene vial) |
| solubility | water: soluble |
| web metadata keyword.default | Extracts, Lysate, Cell Extracts, Tissue Extracts, Bacterial lysate, Electrophoresis, Phosphatase, phosphotyrosine, phosphoserine |
| storage temp. | −20°C |
