General description
CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Immunogen
Human acute lymphoblastic leukemia (ALL) T cells
Biochem/physiol Actions
The mouse monoclonal antibody L17F12 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Physical form
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | PerCP-Cy5.5 conjugate |
| Quality Level | 100 |
| antibody product type | primary antibodies |
| clone | L17F12, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2aκ |
| UniProt accession no. | P06127 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CD5(921) |
